GingaNinga

wallstreet bets whispers in greek…

Joe rogan origin story.

I’ve just given up on multiplayer, its just not fun anymore. I’ve been playing expo 33, blue prince, phantom liberty and lies of P and having a great time. They just don’t make fun multiplayer anymore. If they made something like halo 3 or bad company 2 I’d be back in a heart beat. I think the last multiplayer game I actually got into was rainbow 6 siege and but even that drove me away after year 4-5 or something.

I’ll take indie games overflowing with heart/soul/purpose/ a story to be told any day over cookie cutter tripple A slop.

that thumbnail is gross, that glass evokes whisky and they’re pouring milk into it… its so off-putting lol

slam

I hope 3/4 of the budget goes into smashing pots.

People at war with ancient egypt. Can’t harm cats, they’re sacred. Boom, checkmate!

oh I only listened to the first audiobook up to leto and his showdown with the emperor. I’m out of stupid listening hours on spotify and can’t wait to continue with the rest of them. The books are crazy good so far.

I thought paul had one son in the books…

suppository

Thank you for your service/commitment to the meme!

me too, thanks.

Thanks for the explainer! My lab specializes in IHC so I’m generally familiar with ag-ab interactions but not so much with antibody development. Its fascinating!

To my knowledge its easy to do this with proteins that are directly coded for in our genes. For example there is a gene for insulin so we can clip it, transfect it into a cell culture and get those cells to crank out a bunch of insulin that we can extract, purify and make into a usable, stable product. The problem with antibodies is that we need to be exposed to an antigen capable of stimulating the development of the corresponding antibody. There is a very complicated set of receptors that capture the antigen, internalize and process it to present it to cells that will modify a whole bunch stuff (Thats the limit of my personal knowledge) to generate an antibody capable of neutralizing that substance. In short you still need a living being with some sort of immune system capable of processing that compound and generating an antibody to it, its unbelievably complicated. The good news is once that cell starts cranking out antibody it starts to clone itself so we can extract it, make a hybridoma (fuze the antibody-producing cell from a live animal with immortal cancer cells to create an immortal hybrid that cranks out the desired antibody) then we can continue with the process in cell culture as per usual. Its just cheaper and easier to use live animals with high titre to that antibody. Source: I’ve taken a couple immunology courses and my lab specializes in immunohistochemistry so I’m quite familiar with antigen/antibody interactions but not so much with antibody production.

The immune system is super complicated but essentially when an animal or person are exposed to some sort of substance white blood cells sample that compound and create an antibody that is tailored to bind and neutralize it. Different substances range in their ability to induce this response and it often takes repeat exposures to get the titre to high enough levels. So one way or another some sort of animal needs to be exposed to the venom whether it be horse, goat or mouse and we would harvest the antibody for use in emergency situations where we need to immediately reverse the effects of a snake bite.

Another way would be to do this in cell culture but I’m not quite sure how that works, I think thats easier to do when the proteins are directly coded for like insulin or something. You could probably harvest B cell clones that produce the antibody to the venom, make them immortal and harvest it that way but I’m less familiar with that sort of wok so I can’t really comment further.

Information is just a black hole now, I tried to ignore it too but its impossible to escape. These terrible decisions impact the entire world, its better to know than to not.

NICE!

While the seat is off I recommend installing a bidet, they hook up right to the water at the back of the toilet. 10/10 recommend.

It was Skill-up’s review that turned me off it and I’m glad I never played it from the rest of the reviews I’ve read. The art direction is so off-putting and I love the line “it feels like HR is in the room with you in every scene”, I have no idea what they were thinking.